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Biochemist 1
Ref No.: 18-18915
Location: San Diego, California
Job Title: Biochemist 1, Scientific Operations
Location: San Diego ,California,92121
Duration: 12 Months

  • The Biochemist I is expected to work independently, perform all laboratory functions that involved in Tissue Culture such as Hybridoma roller bottle cell culture, ascites production, insect cell shaker culture, recombinant protein production, in-process assays and safety procedures.
  • The Biochemist I is expected to learn sterile technique and make antibodies.
  • The biochemist I is expected to be capable to complete assigned tissne culture procedures with good quallty by following SOPs and batch records.
  • The posi tion is expected to perform data entries timely and properly.
  • The biochemist I is expected to learn more procedures regularly based on the production requirements.
  • The position is expected to finish assignments on time to help keep up Tissue Culture productivity.
  • In addition, biochemist I is responsible for completing their individual training plan based on the approved IIGs annually.
  • The position works closely with senior biochemist to ensure that the assignments arc done properly before due dates.
  • The position will support and participate in continuous improvement projects as identified by the Executive Steering Committee and assigned by the manager.

Position Qualifications
  • Education: Typically requires BS degree at a 4 year degree program in Science or equivalent combination of related education md experience.
  • Work Experience: Typically requires 0-2 years' experience in a Biotech/ Life Science manufacturing environment.
  • Have some working knowledge in the science disciplines and operation of basic lab equipment.

Techniques Required:
  • Pipetting
  • Dilution
  • Centrifugation
  • Sterile technique Techniques
  • Cell culture

Knowledge and Skills Required:
  • Demonstrated ability to use laboratory instruments and applicable computer software.
  • Knowledge of general laboratory safety
  • Effective interpersonal skills, verbal and written communication
  • Demonstrated ability to follow established policies and procedures.
  • Demonstrated ability to keep neat, accurate, complete and legible records and logs.
  • Possess analytical problem-solving skills.
  • Must be. able to lift a minimum of 25 poui1ds weight.

Physical Requirements:
  • General physical requirements: Sedentary work
  • Visual acuity requirements: Inspection / Administrative.
  • Motion and sensory requirements: Talking, hearing and walking.

Principal accountabilities:
  • Implement and follow all new and current department policies and procedures per departmental SOP's and Batch Records for all manufacturing functions within the department.
  • Follow all departmental guidelines and procedures with minimal Departmental Supervision.
  • Maintain all manufacturing batch record
  • Be able to perform data entry in all required data bases
  • Other duties as assigned by Supervisor.

60-80% Operations I 40-20% technical training
  • Responsible for learning the sterile Tissue Culture technique to independently work under Biosafety hood.
  • Follow the Tissue Culture SOP to make different kinds monoclonal antibody and cell related products by Hybridoma roller bottle cell culture and ascites production and other mammalian cell related products.
  • Follow the Tissue Culture SOP to perform insect cell shaker bioreactor for producing recombinant protein by using Baculoviral or E.coli expression system.
  • Complete Batch. Records accurately and in a timely manner.
  • Follow all SOP's and Work Instructions precisely
  • Perform clarification ion and <>concentrator 1of Tissue Culture supernatant using the Tangential Flow system and or centrifugation.
  • Perform in-process assays Nephelometer, Mycoalert and tsotype ELISA.
  • Help maintain the cell bank inventory by accurate retrieval and placement of vials from L:>l "2freezer.
  • Promote a safe working environment. Follows BO Biosciences Environmental l, Health and Safety (EH&S) po I iciest and procedures. Takes responsibility for safety in. immediate work area. Participates- in EH&S programs. Notifies supervisor-s of all obscr\lcd hazardous conditions or unsafe work practice-es, May. provide recommendations On maintaining the safety of the work environment.
  • Perform other related duties and assignments as required.